Product Introduction

The fluorecare® 25-OH Vitamin D Test Kit (25-Hydroxyvitamin D, 25-OH-D) developed by Microprofit is a quantitative immunofluorescence assay (FIA) designed for the accurate detection of total 25-hydroxyvitamin D in human serum and plasma. As a key biomarker for assessing vitamin D status, 25-OH-D plays a critical role in bone health, calcium metabolism, and immune function.

As part of the fluorecare® POCT diagnostic platform, this test kit enables rapid, reliable, and quantitative analysis, supporting timely clinical decision-making in hospitals, laboratories, and decentralized point-of-care settings.

Detection Principle

The fluorecare® 25-OH Vitamin D test kit utilizes fluorescence immunochromatographic assay technology. In the test process, 25-OH vitamin D in the sample binds to specific fluorescent-labeled antibodies, forming antigen-antibody complexes. These complexes are captured on the test line, generating a fluorescence signal.

The signal is detected and quantitatively analyzed by the fluorecare® immunofluorescence analyzer, enabling precise measurement of 25-OH-D concentration in the sample.

Clinical Applications

• Evaluation of vitamin D deficiency and insufficiency
• Monitoring vitamin D supplementation and therapy
• Assessment of bone metabolism and osteoporosis risk
• Screening in high-risk populations (elderly, pregnant women, chronic disease patients)
• Support for calcium and phosphorus metabolism analysis
• Clinical laboratory and decentralized POCT testing

Frequently Asked Questions

What is the fluorecare® 25-OH Vitamin D test kit used for?
The fluorecare® 25-OH Vitamin D test kit is used for the quantitative detection of 25-hydroxyvitamin D to evaluate vitamin D status and support diagnosis of deficiency-related conditions.

How fast is the fluorecare® 25-OH Vitamin D test?
The test provides accurate quantitative results within 15 minutes when used with the fluorecare® immunofluorescence analyzer.

What samples can be used for this test?
Serum and plasma samples are suitable for testing with the fluorecare® 25-OH Vitamin D test kit.

Is the fluorecare® 25-OH Vitamin D test suitable for POCT use?
Yes, it is specifically designed for point-of-care testing environments as well as centralized laboratories.

Compatible Analyzer

The fluorecare® 25-OH Vitamin D test kit is specifically designed to work with the fluorecare® immunofluorescence analyzer, delivering fast, accurate, and quantitative results. This integrated POCT solution ensures streamlined workflow and reliable performance across laboratories, clinics, and decentralized healthcare settings.

• Quantitative detection based on immunofluorescence assay (FIA)
• Rapid results within 15 minutes
• High sensitivity and specificity for 25-OH vitamin D
• Seamless integration with fluorecare® POCT platform
• Stable performance with internal calibration
• User-friendly operation with minimal training required
• Suitable for laboratory and point-of-care testing scenarios